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PDF: Highly Specific and Quantitative Gene Expression Profiling Based ...

Download 2 times Source: www.jsbi.org // Topic: Biology File Size: 98.02 kb // Total of Page: 2 Page(s) Last download by constantin2378 Last download on: 10 months ago Share this info: Sample of page (click to enlarge) Short Desciption: Here, we introduce a gene expression profiling method based on DNA computing, ... seven target gene DNA oligomers were subjected to the DNA computing for ... Content Inside: Genome Informatics 12: 259260 (2001) 259 Highly Sp ecific and Quantitative Gene Expression Profiling Based on DNA Computing Nao Nishida1 nishida@genta.c.u-tokyo.ac.jp Masatoshi Wakui2 wakuim@medicine.ufl.edu Katushi Tokunaga2 tokunaga@m.u-tokyo.ac.jp 1 2 Akira Suyama1 suyama@dna.c.u-tokyo.ac.jp Department of Life Sciences, Graduate Scho ol of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan Department of Human Genetics, Graduate Scho ol of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan Keywords: DNA computing, gene expression, universal DNA chip 1 Introduction DNA chips and microarrays have been used to simultaneously analyze the expression profiling of many genes [1, 2]. In the analysis, the two-color method [3] is generally employed to determine the relative expression levels between two samples. However, not only the relative expression levels between target genes in a sample but also the absolute expression level of each target gene in a sample cannot be determined accurately with conventional DNA chips and microarrays combined with the two-color method. This hinders extensive and quantitative analysis of gene expression